KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme

KOD Xtreme™ Hot Start DNA Polymerase | 71975. KOD Xtreme™ Hot Start DNA Polymerase Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates. - Find MSDS or SDS, a COA, data sheets and more information.

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Kod xtreme hot start

The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). Shop a large selection of products and learn more about MilliporeSigma™ Novagen™ KOD Xtreme™ Hot Start DNA Polymerase . KOD XTREME HOT START DNA POLY. MFRPN: 71975-3: SKU: VWR95042-700-EA: Weight: 1.0000: Shipping Estimate: Please login for price In parallel with the study of the activities of KOD DNA polymerase, neutralization antibodies for the polymerase and proofreading activities were developed (2). These antibodies can be applied to the "hot start PCR technology" using KOD DNA polymerase.

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Produktkod. 71975. REACH- registreringsnummer. : Denna produkt är en blandning.

KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content.

Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. We offer different hot-start DNA polymerases to support your everyday research needs. Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl 2, KCl and stabilizers.It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. Jednoręki bandyta Xtra Hot to propozycja zarówno dla doświadczonych graczy szukających rozgrywki na poziomie, i również dla początkujących o małej wiedzy na temat gier wrzutowych.

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Ideal for amplifying crude samples with minimal sample preparation, this kit translates into less reagents and less time spent on PCR. Article Snippet: The mix for KOD Xtreme™ Hot Start DNA Polymerase was performed using 2.4 μL of distilled water, 10 μL of 2X reaction Xtreme buffer, 4 μL of dNTPs (200 μmol/L each), 0.3 μmol/L each primer, 0.4 μL KOD Xtreme Hot Start DNA polymerase, and 2 μL of DNA template. KOD Xtreme™ Hot Start DNA Polymerase - Free download as PDF File (.pdf), Text File (.txt) or read online for free. KOD Xtreme™ Hot Start DNA Polymerase specimens. The KOD Xtreme™ Hot Start DNA Polymerase system’s unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification.

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Six polymerases shown were used to amplify a 90% GC-containing region of human IGF2R [NM_000876] from HeLa cell cDNA. KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided.

Utilises a DNA polymerase with a low error rate Amplifies directly from minimally processed samples KOD Hot Start DNA Polymerase is a premixed complex of the high fidelity KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (1). KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of May 14, 2008 – To address the needs of scientists working with challenging PCR targets, EMD (in partnership with Toyobo Co.) announces the release of the Novagen® brand KOD Xtreme™ Hot Start DNA Polymerase kit (not available in Japan). KOD Xtreme polymerase is an optimized PCR system for the amplification of long or GC-rich DNA templates. The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). KOD -Plus- generates blunt-end PCR products, due to 3'→5' exonuclease (proof-reading) activity.

Description The KOD Xtreme Hot Start DNA Polymerase kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The system includes an ultra high fidelity KOD DNA Polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with …Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA… KOD Xtreme™ Hot Start DNA Polymerase Show Pricing & Availability Catalogue Number Availability Packaging Qty/Pack Price Quantity; 71975-3: Retrieving availability KOD Xtreme™ Hot Start Polymerase amplifies GC-rich targets more efficiently than other polymerases. Six polymerases shown were used to amplify a 90% GC-containing region of human IGF2R [NM_000876] from HeLa cell cDNA.